total flavonoids extracted from xiaobuxin-tang on the hyperactivity of hypothalamic-pituitary-adrenal axis in chronically stressed rats总类黄酮提取的多动症xiaobuxin-tang肾上腺轴在长期强调老鼠.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 367619, 7 pages doi:10.1093/ecam/nep218 Original Article Total Flavonoids Extracted from Xiaobuxin-Tang on the Hyperactivity of Hypothalamic-Pituitary-Adrenal Axis in Chronically Stressed Rats Lei An,1, 2, 3 You-Zhi Zhang,1 Xin-Min Liu,3 Neng-Jiang Yu,1 Hong-Xia Chen,1 Nan Zhao,1 Li Yuan,1 and Yun-Feng Li1 1 Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China 2 College of pharmacy, Dalian Medical University, Dalian 116044, China 3 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100094, China Correspondence should be addressed to You-Zhi Zhang, zhyouzhi@ and Xin-Min Liu, liuxinmin@ Received 15 April 2009; Accepted 25 November 2009 Copyright © 2011 Lei An et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Our previous studies have demonstrated that the total flavonoids (XBXT-2) isolated from the extract of Xiaobuxin-Tang (XBXT), a traditional Chinese herbal decoction, ameliorated behavioral alterations and hippocampal dysfunctions in chronically stressed rats. Studies over the last decades have suggested that the hyperactivity of hypothalamic-pituitary-adrenal (HPA) axis is one of the most consistent findings in stress-related depression. Herein, we used the same chronic mild stress model of rats as before to further investigate the effect of XBXT-2 on the hyperactivity of HPA axis, including the stress hormones levels and glucocorticoid receptors (GRs) expression. Our ELISA results showed that chronic administ