黄芪治疗荨麻疹机制实验探究.docVIP

黄芪治疗荨麻疹机制实验探究[摘要] 目的:观察黄芪对大鼠被动皮肤过敏反应(PCA)的抑制作用,初步探讨其治疗荨麻疹的可能机制。方法:建造同种大鼠PCA模型,实验动物随机分为模型组、酮替酚组、黄芪组,分别给予相应药物灌胃给药,采用HE染色法、甲苯胺蓝染色法、分光光度计比色法观察大鼠PCA及肥大细胞脱颗粒情况。结果:黄芪可减轻大鼠PCA,减少肥大细胞脱颗粒。结论:黄芪对大鼠PCA有抑制作用,其可能的作用机制为抑制肥大细胞脱颗粒,减少炎症介质的释放。 [关键词] 黄芪;荨麻疹;被动皮肤过敏反应;抑制作用;肥大细胞脱颗粒 [中图分类号] R758.24[文献标识码]A [文章编号]1673-7210(2010)03(c)-033-02 Experimental study of mechanism of Astragalus in treating urticaria WU Lingxia, ZHEN Li (Department of Dermatology, the First Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China) [Abstract] Objective: To investigate the effects and mechanism of Astragalus on urticaria by using experimental animal model of PCA. Methods: Rat models of PCA were established, then the rats were randomly divided into model group, Ketotifen group and Astragalus group. Corresponding medicines were injected into their stomachs to carry on the experiment. The PCA and degranulation of mast cells were observed with HE, toluidine blue and spectrophotometer. Results: Astragalus could decrease the PCA reaction, and decreased the degranulation of mast cells. Conclusion: Astragalus can decrease the PCA reaction, and its possibled mechanism is decreasing the degranulation of mast cells and reducing production of active mediator. [Key words] Astragalus; Urticaria; PCA; Inhibition; Degranulation of mast cell 黄芪(Astragacus,AS)是多年生豆科植物蒙古黄芪或膜荚黄芪的干燥根[1],始载于《神农本草经》,以补中益气、升举清阳为主要功效,是补气的要药[2]。我科运用黄芪治疗荨麻疹疗效确切,但其作用机制尚不清楚。本实验旨在进一步观察黄芪对大鼠被动皮肤过敏反应(PCA)的抑制作用,初步探讨其可能的机制,从而为临床用药提供依据。 1 材料与方法 1.1 实验动物 Wistar大鼠35只,体重(160±20) g,雌雄兼用,购自山西医科大学生理实验室,分笼饲养于山西医科大学生理实验室。 1.2 药物与试剂 卵清蛋白(Sigma公司,批号A5253),白百破疫苗(武汉生物制品研究所,批4),黄芪(豪州市万珍中药饮片厂,批号081226),富马酸酮替酚片(海南制药厂有限公司制药一厂,批号081101),伊文思蓝,甲苯胺蓝。 1.3 仪器 低速离心机SC-3610(科大创新股份有限公司中佳分公司),721型分光光度计(上海第三分析仪器厂)。 1.4 实验方法 1.4.1 动物分组Wistar大鼠30只,随机分为模型组、酮替酚组、黄芪组。模型组:生理盐水2 ml/只;酮替酚组:0.7 mg/kg;黄芪组:5 g/kg。 1.4.2 大鼠同种PCA模型的构建及处理各组药物均配制成2 ml溶液,灌胃给药,每天1次,连续7 d,第5天给药后在大鼠背中线两侧选取致敏点。取卵蛋白IgE抗血清生理盐水溶液(1∶1),于皮内注射0