从琼脂糖电泳凝胶中回收DNA的几种简便方法.PDF

3 2 生命科学研究 V o l . 3 N o. 2 1999 6 L if e Science Research Jun . 1999 : 1007 -7847 ( 1999 02-0128-05 DNA a 王利民, 唐建国 ( , 100871 : 介绍两类从普通琼脂糖电泳凝胶中回收DN A 的简 、快捷、高效且廉价的方法. 第 一类为电泳洗脱法. 方法 a: 利用 1. 5 mL 微量离心管、l mL 吸头、尼龙网膜和透析膜做成的 一个小装置, 快速有效回 , 最终回收率为70 % 左右. 方法 : 不用 - 纤维素膜, 而 DN A b D EAE 用透析膜在凝胶中作出横隔挡在DN A 条带前, 最终回收率为50% 左右; 第二类为冰冻融解 法, 最终回收率也在50% 左右. 如果联合使用冰冻融解法和电泳洗脱法, 回收率可进一步提 高至90 % . : 琼脂糖凝胶电泳; 电泳洗脱; 冰冻融解; D NA 回收 : Q 78 1: A Several Convenient Methods of Recovering DNA from Agarose Electrophoretic Gels - , - WA N G L i m in T A N G Jian gu o ( Co llege o f L if e Sciences, Beijing U niver sity , Beijing 100 87 1, China : , , Abstract T w o cat eg ories of easy rapid eff ect iv e and cheap m et hods f or reco ver ing D NA fr om agarose g el elect ropho retic g els ar e repor ted . T he f irst cat eg ory is elect ro elut ion . M et hod a: A r ecovering unit w as made by u sing 1. 5 mL m icrocent rif ug e t ube, l m L t ip , nylon web m em br ane and dialysis membrane. T he f inal reco ver y rat e of this rapid and ef - fect ive method co uld be raised t o 70 % . M ethod b : A sl it w as cut in t he g el im mediat ely a- head of t he D NA f ragm ent ( s of int erest and a sliver of dialysis membrane, instead of DEA E-cellulose membrane, was insert ed int o t he slit . T he recov ery r at e o f t his elect roelu - tio n m et hod was about 50 % . T he seco nd cat egory is t he freezing t ha