生物分离工程考试秘籍（Bio Separation Engineering Examination Cheats）.doc

生物分离工程考试秘籍（Bio Separation Engineering Examination Cheats） The separation of 1. biological products includes removal_of, insolubles, isolation, purification, polishing, four steps. 2. methods of solid-liquid separation of fermentation liquid are commonly used: _ __ centrifugal ______ and _ filter _______ two. 3. centrifugal equipment from the form can be divided into _ tubular sleeve type _, _ _, _ disc type _ three types. 4. affinity adsorption principle including _ adsorption medium _ preparation, adsorption and elution _ _ _ _ three step. 5. polysaccharide based ion exchange agent including _ exchange agent and _ glucose molecules _ ion exchange cellulose _ two categories. Ultrafiltration device 6. commonly used on the industrial _ plate _, _ tube _, spiral wound _ and _ hollow fiber type __. There are 7. main factors affecting the adsorption properties of _ adsorbent, adsorbate properties _ _, _ _ _ _ temperature, pH and salt concentration, _ adsorbate concentration and adsorbent dosage _ _ _. 8. ion exchange resin by _ carrier _, active group _ and __ exchangeable ion __ composition. Prepared by gel electrophoresis 9., ammonium persulfate is _ initiator _; methylene bisacrylamide is the role of crosslinking agent TEMED is _; _ accelerator _; 10 effects of salting out factors of solute species _ _, _ _ solute concentration, _PH value and temperature _ _ _; 11. according to the separation mechanism of different, can be divided into _ adsorption chromatography, chromatography chromatography, ion exchange chromatography and gel chromatography _. In the experiment 12. salting out Cohn equation for solute solubility associated with salt concentration for _lgS= beta -KsI2_; when the system temperature, pH value _ keep unchanged, only change the ionic strength _ salting out operation called Ks salting out; when the ionic strength _ remain unchanged, changes in temperature, pH of the salting out operation _ called beta salting out method. 13. the formation of supersatura