第五章 细胞融合（The fifth chapter is cell fusion）.doc

第五章 细胞融合（The fifth chapter is cell fusion） In the fifth chapter, cell fusion.Txt can be put up and put down, it is called weight lifting. If you can hold something up and can not put it down, it is called weight lifting. The head should have courage, and rise to have the spirit. Learn to add, pride to subtract, opportunity to ride, sloth to divide. The three problems of life: thinking, love, Unrequited love. The fifth chapter is cell fusion (cell fusion) I. The concept of cell fusion The technique of artificially combining two or more cells of the same or different species into one cell by mediating the action of the two. Also called somatic cell hybridization (somatic, hybridization) Two, the meaning of cell fusion (application) 1 gene mapping and mapping of human genes Gene targeting method Pedigree analysis Cell fusion Gene dosage effect method Recombinant DNA method Molecular hybridization Restriction fragment length polymorphism Pulse electrophoresis (PEGE) Genetic map: a map that maps all genes on a human chromosome in detail, in order, and at intervals, also called chromosome linkage maps 2 induce PC chromosome Tumorigenicity of 3 somatic hybrids 4 genetic complementation of genetic defects Study on expression regulation of 5 differentiated functions 6 lymphocyte hybridoma technique and monoclonal antibodies Cloned cells, antibodies produced by the proliferation of a hybridoma cell secreting called highly pure monoclonal antibody 7 plant protoplasts were fused to form hybrid plants 8 nuclear cytoplasmic interaction 9 gene therapy 10 disease diagnosis Three, the mechanism of cell fusion Membrane fusion is the interaction and fusion of two different membranes, resulting in the mixing of two membrane lipids and membrane proteins and mixing of both sides of the inclusion. The basic steps of cell fusion include: 1 cells are close to each other 2 cell bridge formation 3 cytoplasmic penetration 4 nuclear fusion By various fusion mechanism 1) fat disorder model Lucy,