适当的展开剂（Proper spreading agent）.docVIP

适当的展开剂（Proper spreading agent） Choose the appropriate expansion agent is the primary task. The commonly used solvent according to order of increasing polarity probably: Oil many hexane ether THF benzene ethyl acetate acetone ethanol methanol use single solvent, often can not achieve a good separation effect, often using mixed solution mixed solvents usually use a high polar and low solvent composition, high polar solvent and increase the discrimination effect of combinations of solvents are commonly used: Petroleumether/Ethylacetate, petroleumether/Acetone, Petroleumether/Ether, Petroleumether/CH2Cl2, ethylacetate/MeOH, CHCl3/ethylacetate The proportion of the agent depends on the general attempt. According to the literature reported in the compounds with what kind of the agent, is the first attempt to use this kind of expansion agent, and then try the proportion continuously, until you find a good separation effect of expansion agent. Expansion agent: the selection condition of the desired composition has good solubility; II can make the components separately; the component to be tested in Rf 0.2~0.8, quantitative determination in 0.3 ~ 0.5; II with analytes or adsorbent chemical reaction; the moderate boiling point, viscosity is small after the start of the component; and the circular spots concentrated; the best mixed solvent with freshly prepared. Generally speaking, the basic two-phase weakly polar solvent system by n-hexane and water, then according to the needs of methanol, ethanol, ethyl acetate to regulate the polarity of the solvent system, in order to achieve good separation effect, suitable for the separation of alkaloids, terpenoids, Huang Tong etc.; medium polar solvent system by chloroform and the water phase, composed of methanol, ethanol, ethyl acetate, etc. to adjust, suitable for anthraquinone, coumarin, and some of the more polar lignans and separation of terpenoids; strong polar solvent, composed of n-butanol and water, but also by methano