高三一轮复习——动物细胞培养和核移植技术-30（A review of senior three -- animal cell culture and nuclear transfer technology -30）.docVIP

高三一轮复习——动物细胞培养和核移植技术-30（A review of senior three -- animal cell culture and nuclear transfer technology -30） The Zhang Bei learning plan system is shared every day Two. Study content: The process of animal cell culture in this week should be distinguished from animal cell culture and plant tissue culture, and the process and significance of cell nuclear transfer in animals will be mastered Three. Study the key points and difficulties Understanding the process of animal cell culture. To understand the principle of somatic cell nuclear transfer technique. Four. Learning process: (I) learning content 1. animal cell culture: cell culture refers to the growth of cells in vitro, and the animal cells are no longer formed in the process of culture. Concept: extract tissue from an animals body, disperse it into a single cell, then place it in a suitable medium to allow the cells to grow and proliferate. Components of the culture medium: glucose, amino acids, inorganic salts, vitamins, animal serum, etc.. Animal cell culture is the key to success in the culture medium containing animal serum, because the internal environment of animal cells life and some components have not been studied clearly, so it is necessary to add animal serum to provide a similar organism environment, in addition in animal serum also contains some animal hormones and enzymes, can promote cell growth. Characteristics of culture medium: liquid medium, animal serum. Culture conditions: sterile, non-toxic environment; nutrients (synthetic medium); temperature and pH (+ C ~); gas (oxygen and carbon dioxide incubator, mixed gas in animal cell culture dishes or loose cover bottle placed in the 95% air and 5% CO2 in the training.) Etc.. Basic process: Take animal embryos or young animal organs and tissues. The material is cut and treated with trypsin (digestion) to form a single cell. The individual cells are placed in the medium and assigned to a certain concentration of cell suspension. The dispersed cells