naja mitochondrial genome sequence analysis（眼镜蛇线粒体基因组序列分析）.docVIP

Naja mitochondrial genome sequence Analysis [Abstract] [purpose] 2 Zhoushan Cobra’s mitochondrial gene group full sequence carried out a measured and analyzed, and preliminary explore its system development status. [Method] combination of PCR product was TA cloned and step-by-step shift sequencing technology, based on the addition to nad6 outside the 12 Ge merge sequence and its translated amino acid sequence of the protein-coding genes, lizards outgroup Neighbor Joining (NJ) method to build phylogenetic trees of seven species of snakes. [] both in length only difference between the 2 bp gene exactly the same content and sort encodes 37 genes (two rRNA, 22 tRNA, and 13 protein), which tRNAGln tRNAAla, tRNAAsn tRNACys #8203;#8203;tRNATyr and tRNASer (UCN), tRNAGlu, tRNAPro and nad6 from light (L ) chain encoded by heavy (H) chain coding .13 ranking of protein-coding genes and the relative position is fully consistent with the other snakes, the start codon of cox1 use GTG, the rest are ATG; at the same time analysis found that, in addition to outside nad1/4L/5, cox1 and atp8 are not completely terminate the codon TA or T.NJ of tree Elapidae and Colubridae closest genetic relatives, followed with the, and pythons Division, Viperidae poly with the support of each branch are up to 90% or more. [Conclusion] This study confirmed the sequence based on the composition of the mitochondrial genes and phylogenetic studies of snakes species is feasible. [Words] Naja; genomics; controlled area; phylogeny; mitochondria Naja, also known as the Chinese cobra, Latin, called “Naja atra”, the English name “Zhoushan cobra”, “Taiwan cobra” or Chinese cobra “, its affiliated Reptilia (Reptilia) Squamata (Squamata) Snake suborder (Serpentes) Elapidae (Elapidae) Cobra case (Naja), are mainly located in the southern part of the country and the island of Taiwan and northern Vietnam was listed as in the Convention on International Trade lt;lt; Endangered Species (CITES) gt;gt; appe