如何让学生爱上你的语文课（How to let the students fall in love with your Chinese lesson）.docVIP

如何让学生爱上你的语文课（How to let the students fall in love with your Chinese lesson） Recombinant inclusionbody protein refolding Zouping The development of genetic engineering technology has opened a new chapter of human life science, has opened a new era of modern Bio Industry development. Recombinant DNA technology for the large-scale production of the target protein may be provided, E.coli for its easy operation, clear genetic background, the expression and protein level of low fermentation cost advantages, is the preferred expression system for recombinant protein production. But the high expression of exogenous gene in E.coli often leads to the formation of inclusion bodies, how efficient refolding is a difficult problem of genetic engineering technology. With the implementation of the completion of the human genome and proteome, there will be more people to face this challenge. A, spheroidins 1, the formation of inclusion bodies The inclusion body is mainly because of the lack of a cofactor for certain protein folding process in the expression of recombinant protein, and can not form correct secondary bonding and other reasons; it may be exogenous gene synthesis speed is too fast, there is not enough time for nonspecific folding, two disulfide bonds cannot correct pairing, too much protein the combination of protein, can not reach the enough solubility; the primary structure of recombinant proteins and inclusion body formation, generally more sulfur amino acid is easy to form inclusion bodies, and the content of proline was significantly associated with formation of inclusion bodies were positively correlated; recombinant protein environment discomfort, high fermentation temperature or intracellular pH close to the isoelectric point of the protein is easy to form inclusion bodies. 2, reduce the formation of inclusion body strategy Reduce the growth temperature of the recombinant strain is the most commonly used method to reduce the formation of inclusion body. Low grow