使用夹心TTC染色法可显着提高脑缺血模型中梗死灶的检测效率.PDFVIP

4 J Apoplexy and N ervous D iseases, January 2011, Vol 28, No. 1 : 2011) TTC 蒋 斌, 张成岗 : (TTC), Bal /c(MCAO), 5h, TT C TTC, , TTC , , TT C, : ; ; : R331; R742 : A P rom oting the de tection efficien cy of focal infarc tion after cereb ral ischem ia by sandw ich TTC stain ing m ethod JIANG B in, ZHANG ChengGang. (B eij ing Institute of Rad ia tionM edicine, S tateK ey aboratory of Proteom ics, S tateK ey aboratory of M edicalN eurobiology, B eij ing 100850, China) A bstrac t: Obj ec tive In order to evaluate the location and s ize of the focal infarction region in cere ral ischem ia etter, the triphenyltetrazolium ch loride(TTC) stain ing m ethod w as optmi ized. M ethod s After preparation of the m idd le cere ral artery occlusion( MCAO) model in the B al / cm ice, the rainsw ere rem oved after 5h s ischem ia insults. Then sandw ich TT C staining m ethod w as used to determ ine the location and size of the focal infarction. Re su lts Com pared w ith the result w ith only one side of rain slices stainedw ell y traditional TTC staining approach, the sandw ich TTC stai n ing method could m ake oth upper and low er sides of the rain slices stained m uch un iform ly, and easy to identify the fo cal infarction directly. Con clu sion s The optmi ized sandw ich TT C staining approach is a valua le m ethod to determ ine the location and area of focal infarction in the ischem ic rain, andw ill promote the detection efficiency of focal infarction af ter cere ral ischem ia. K ey w ord s: Triphenyltetrazolium chloride; Cere ral ischem ia; Focal infarction , 0. 20mm, )2, 3, 5 (TTC)( 9%, ,