临床1α,25(OH)2D3对兔角膜碱烧伤角膜LC影响的实验探究.pdfVIP

摘 要 目的：研究 1,25 二羟基维生素 D3 （骨化三醇）对兔角膜碱烧伤后角膜朗格罕氏细胞分 布的影响，并初步探讨其作用机制。 方法：实验分为实验组，对照组和正常组，实验组和对照组使用 1M 的NaOH 溶液在兔 角膜制作碱烧伤模型，然后实验组局部和全身给予 1,25 二羟基维生素 D3 的灭菌纯花生油溶 液，对照组局部和全身给予灭菌纯花生油溶液，正常组不做任何处理。分别在第 3，7，21 天时对正常组，实验组和对照组家兔行手术显微镜下观察，角膜共聚焦显微镜，HE 染色观 察角膜病理改变。 结果：正常组角膜中央在三个时间点均未检测出朗格罕氏细胞。实验组和对照组碱烧伤 后 3、7 天角膜中央出现朗格罕氏细胞，对照组密度高于实验组（P0.05 ）；碱烧伤后 21 天 两组朗格罕氏细胞密度相近(P0.05) 。实验组炎性反应程度在第7，21 天时轻于对照组。 结论：角膜碱烧伤早期即可出现朗格罕氏细胞向角膜中央移动；1,25 二羟基维生素 D3 能够在兔角膜碱烧伤早期抑制朗格罕氏细胞的迁移，并且能在一定程度上抑制炎性反应。 关键词：角膜碱烧伤； 1,25 二羟基维生素 D ；朗格罕氏细胞 3 论文类型：B (应用研究) Abstract Objective: To investigate the role of 1,25-dihydroxyvitamin D3(calcitriol) on Langerhans cell in rabbit corneal alkali burn and preliminary study its mechanism action. Methods: 35 rabbits were divided into experimental group, control group and normal group. In experimental group and control group the models of corneal alkali burn were induced in rabbit with 1M NaOH, then administer the experimental group pure peanut oil solution of 1,25-dihydroxyvitamin D3 in local and general way, administer the control group Pure peanut oil solution in local and general way, and normal group without any treatment. Corneas of normal group，experimental group and control group were examined general change surgery under the surgical microscope at the time of day 3, 7 and 21, analyzed corneal pathological changes by corneal confocal microscopy and H-E staining. Results: None Langerhans cell was found in the corneas of normal group on all three days. Less Langerhans cells were found in the experimental group than in the control group on day 3 and 7 (p0.05). Quantity of Langerhans cells in both experiment group and control group was no difference on the day 21 (p0.05). The inflammatory reaction degree of experimental group is slighter than the control group on the day 7 and 21. Conclusions: