刺五加对重症急性胰腺炎肺损伤大鼠核转录因子-κB影响.docVIP

刺五加对重症急性胰腺炎肺损伤大鼠核转录因子-κB影响[摘要] 目的:探讨刺五加对重症急性胰腺炎(SAP)肺损伤大鼠肺组织中核转录因子-κB(NF-κB)的作用。方法:经胆胰管逆行注射5%牛磺胆酸钠制作SAP模型;分3个时间点处死大鼠,取肺组织检测NF-κB水平;光镜观察大鼠肺组织病理变化。结果:刺五加能减轻SAP大鼠肺组织的病理性损伤,降低肺组织中NF-κB水平。结论:刺五加能抑制NF-κB的表达,对SAP肺损伤有保护作用。 [关键词] 刺五加;胰腺炎;肺损伤;核转录因子-κB [中图分类号] R-33 [文献标识码]A [文章编号]1674-4721(2010)03(a)-013-02 The effect of acanthopanax senticosus on nuclear factor-κB in rats with lung injury associated with severe acute pancreatitis CHEN Bo (Laboratory of Physiology Science, Guangdong Medical College, Dongguan 523808,China) [Abstract] Objective: To explore the effects of acanthopanax senticosus on nuclear factor-κB(NF-κB) in rats with lung injury associated with severe acute pancreatitis(SAP). Methods: The model of SAP was performed by injecting 5% sodium taurocholate. Three,six and twelve hours after model establishmeng, the rats were sacrificed in the respective group. Morphologic changes of lung was observed under light microscopy. Results: In acanthopanax senticosus group, lung injury was markedly reducesd,the NF-κB level in lung tissues were decreased significantly. Conclusion: Acanthopanax senticosus can inhibit the expression of NF-κB in lung tissues of rats with SAP, prevent and treat the lung injury by SAP. [Key words] Acanthopanax senticosus; Pancreatitis; Lung injury; Nuclear factor-κB 重症急性胰腺炎(severe acute pancreatitis,SAP)是一种既有胰腺本身病变,又极易并发远隔脏器损害的急腹症。其中肺损伤是SAP早期常见的并发症和死亡原因,其死亡率高达50%[1]。尽管目前关于SAP肺损伤的发病机制还未完全阐明,但近年研究表明,核转录因子-κB(NF-κB)活化及炎症因子的过度表达造成细胞因子网络失衡,导致扳机样细胞因子级联反应,在SAP肺损伤发病过程中可能起重要作用[2]。本研究主要探讨刺五加对SAP肺损伤大鼠肺组织中NF-κB的作用。 1 材料与方法 1.1 材料 健康Wistar大鼠,由广东医学院实验动物中心提供;牛磺胆酸钠为Sigma公司产品;刺五加注射液(acanthopanax senticosus injection,ASI)为黑龙江完达山制药厂生产,批号每支20 ml;RPMI1640培养基、胰蛋白酶为Gibco公司产品;TransAMTM NF-κB p65化学发光ELISA试剂盒购自Active Motif公司;CO2培养箱(美国Forma Scientific);Centrol LB 960型微孔板化学发光检测仪(德国Bethold公司)。 1.2 方法 1.2.1 大鼠SAP模型的建立[3]大鼠术前12 h禁食禁水,以20%氨基甲酸乙酯1 mg/kg腹腔注射麻醉,无菌条件下经腹壁正中切口入腹,除假手术组只翻动胰腺后关腹外,其余两组均在主胰管内逆行插管,注入5%牛磺胆酸钠10 ml/g后结扎主胰管,缝合腹壁。