a quantified ginseng (panax ginseng c.a. meyer) extract influences lipid acquisition and increases adiponectin expression in 3t3-l1 cells量化人参(人参c.a meyer)提取影响脂质收购并增加脂联素表达3 t3-l1细胞.pdfVIP

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a quantified ginseng (panax ginseng c.a. meyer) extract influences lipid acquisition and increases adiponectin expression in 3t3-l1 cells量化人参(人参c.a meyer)提取影响脂质收购并增加脂联素表达3 t3-l1细胞.pdf

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a quantified ginseng (panax ginseng c.a. meyer) extract influences lipid acquisition and increases adiponectin expression in 3t3-l1 cells量化人参(人参c.a meyer)提取影响脂质收购并增加脂联素表达3 t3-l1细胞

Molecules 2011, 16, 477-492; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article A Quantified Ginseng (Panax ginseng C.A. Meyer) Extract Influences Lipid Acquisition and Increases Adiponectin Expression in 3T3-L1 Cells Chia-Rou Yeo, Chen Yang, Ting-Yan Wong and David G. Popovich * Department of Chemistry, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore * Author to whom correspondence should be addressed; E-Mail: chmpdg@.sg; Tel.: +65-6516-4695; Fax: +65-6775-7895. Received: 8 November 2010; in revised form: 21 December 2010 / Accepted: 7 January 2011 / Published: 10 January 2011 Abstract: A Panax ginseng extract (PGE) with a quantified amount of ginsenosides was utilized to investigate its potential to inhibit proliferation, influence lipid acquisition and adiponectin expression in 3T3-L1 cells. Seven fingerprint ginsenosides were quantified using high performance liquid chromatography and their respective molecular weights were further confirmed via LC-ESI-MS analysis from four different extraction methods. Extraction using methanol under reflux produced significantly higher amounts of ginsenosides. The methanol extract consisted of Rg1 (47.40 ± 4.28 mg/g, dry weight of extract), Re (61.62 ± 5.10 mg/g), Rf (6.14 ± 0.28 mg/g), Rb1 (21.73 ± 1.29 mg/g), Rc (78.79 ± 4.15 mg/g), Rb2 (56.80 ± 3.79 mg/g), Rd (5.90 ± 0.41 mg/g). MTT analysis showed that PGE had a concentration- dependent cytotoxic eff