原创力文档a systemic gene silencing method suitable for high throughput, reverse genetic analyses of gene function in fern gametophytes系统性基因沉默法适用于高吞吐量、蕨类植物孢子体的反向遗传基因的分析功能.pdfVIP
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a systemic gene silencing method suitable for high throughput, reverse genetic analyses of gene function in fern gametophytes系统性基因沉默法适用于高吞吐量、蕨类植物孢子体的反向遗传基因的分析功能
BMC Plant Biology BioMed Central
Methodology article Open Access
A systemic gene silencing method suitable for high throughput,
reverse genetic analyses of gene function in fern gametophytes
1 1,2 3
George Rutherford , Milos Tanurdzic , Mitsuyasu Hasebe and
Jo Ann Banks*1,2
Address: 1Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA, 2Purdue Genetics Program, Purdue
University, West Lafayette, IN 47907, USA and 3National Institute for Basic Biology, Okazaki 444-8585, Japan
Email: George Rutherford - gruther@; Milos Tanurdzic - milos@; Mitsuyasu Hasebe - mhasebe@nibb.ac.jp;
Jo Ann Banks* - banksj@
* Corresponding author
Published: 16 April 2004 Received: 02 February 2004
Accepted: 16 April 2004
BMC Plant Biology 2004, 4:6
This article is available from: /1471-2229/4/6
© 2004 Rutherford et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in
all media for any purpose, provided this notice is preserved along with the articles original URL.
Abstract
Background: Ceratopteris richardii is a useful experimental system for studying gametophyte
development and sexual reproduction in plants. However, few tools for cloning mutant genes or
disrupting gene function exist for this species. The feasibility of systemic gene silencing as a reverse
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