guinea pig blood labyrinth barrier permeability in vitro model（豚鼠血迷路屏障通透性体外模型）.docVIP

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guinea pig blood labyrinth barrier permeability in vitro model（豚鼠血迷路屏障通透性体外模型）.doc

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guinea pig blood labyrinth barrier permeability in vitro model（豚鼠血迷路屏障通透性体外模型）

Guinea pig blood labyrinth barrier permeability in vitro model Author: Wei Yunjun, new approach HUANG Mao-hua [Keywords] endothelial cells Keywords: endothelial cells; vitro model; bovine serum albumin; blood labyrinth barrier; guinea pigs Abstract: Objective To study the guinea pig blood labyrinth barrier permeability in vitro model of the establishment of the method and its permeability characteristics. Methods ① tissue blocks adherent culture isolation and culture of the guinea pig cochlea microvascular endothelial cells, immunohistochemical method identified; ② Koike technology established endothelial cell permeability in vitro model to study the model in the cochlea, the permeability of the endothelial cells of the brain and lungs of 125 I-bovine serum albumin; ③ research guinea pig blood labyrinth barrier of 125 I-bovine serum albumin permeability. Results ① cultured endothelial cell culture cell dense fusion typical “cobblestone” appearance by immunohistochemistry was detected by its endothelial cell marker antigen factor Ⅷ, more than 95% of the cultured cells Baker - Time change map showed a parabolic rise curve the brownish yellow positive reaction in the cytoplasm; ② cochlea, added 125 I-bovine serum albumin in vitro model of endothelial cells in the brain and lungs, almost linear 90min similar; ③ guinea pig blood labyrinth barrier permeability curves and in vitro models of the 125 I-bovine serum albumin conclusion vitro model of the blood-labyrinth barrier substantially reflected in the body when the permeability characteristics. Keywords: endothelial cell; model in vitro; bovine serum al-bum; blood labyrinth barrier; guinea pig Abstract: AIM To study the method of setting up model in vitro for the permeability of blood labyrinth barrier from guinea pigs and the model’s permeability.METHODS ① Cochlear microvascular endothelial cells (CMECfrom guinea pigs were isolated and cultured by tissue nubbles culti-vation and were identified with im