hypoxic hippocampal neurons in vitro protective effect of ginsenoside（缺氧海马神经元体外人参皂苷的保护作用）.docVIP

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hypoxic hippocampal neurons in vitro protective effect of ginsenoside（缺氧海马神经元体外人参皂苷的保护作用）.doc

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hypoxic hippocampal neurons in vitro protective effect of ginsenoside（缺氧海马神经元体外人参皂苷的保护作用）

Hypoxic hippocampal neurons in vitro protective effect of ginsenoside : Wang Shuangyan the Wang Shoubiao Shen If Wu Lian Qing Yang Xuewei [Keywords:] ginseng [Abstract] Objective To observe the effect of ginsenosides (Rg2 on hypoxic hippocampal neurons protective effect and its mechanism. Methods of neonatal Wistar rat hippocampal neurons in vitro 14 d were randomly divided into control group, 5 μmol / L nimodipine the amlodipine group (nimodipine group, Rg2, 0.025 mmol / L group (Rg2, 1 group, Rg2, 0.050 mmol / L group (Rg2, group 2 the corresponding drugs added to the culture medium and incubated for 4 h after continuous charge to the volume fraction of 0.95 N2 + volume fraction of 0.05 of CO2 gas mixture, the establishment of the cell model of acute hypoxia. trypan blue staining of surviving cells, Giemsa staining of cell morphology, the fluorescence spectrophotometer to measure the cell [Ca2 +] results nimodipine group, Rg2, two doses group of cell death was significantly lower than the control group, Rg2, 2 were the lowest, the difference were significant (χ 2 = 3.37, P lt;0.05. hippocampal neurons intracellular Ca2 + concentration in the nimodipine group, Rg2, the two dose groups were significantly lower than the control group, Rg2, 2 groups of the lowest, the difference was significant (F = 466.58, q = 6.76 ~ 48.82, P lt;0.01. Conclusion Rg2 can significantly reduce the in vitro hypoxic hippocampal neurons death rate, its mechanism may be through reducing of Ca2 + influx play a role. [Keywords:] ginsenosides, hypoxia, brain, the hippocampus, neurons, calcium [ABSTRACT] ObjectiveTo investigate the neuroprotective effect and its mechanism of Rg2 in anoxic cultured hippocampal neuronal cells in newborn rats. MethodsAnoxic cultured hippocampal neuronal cells of newborn rats were divided into control group, nimodipine group (5 μmol / L), Rg2 1 (0.025 mmol / L) group and Rg2 2 (0.050 mmol / L) group. The activities of the neurons were tested by trypa