雷公藤红素对肝叶部分切除小鼠学习记忆和海马胶质细胞地影响.pdfVIP

徐州医学院硕士学位论文 结论 老年小鼠手术创伤后的学习记忆能力下降，雷公藤红素能改善老年小鼠术后 学习记忆能力的下降， 抑制神经胶质细胞的激活是其可能机制之一。 【关键词】 雷公藤红素；水迷宫 ；胶质细胞； 3 徐州医学院硕士学位论文 Celastrol ’ s role in the cognition and glial cell in hippocampus from mouse whose hepar being partly resected Abstract Objective To research the celastrol ’ s role in cognition function and glial cell in hippocampus from mice,which undergoed a partial resection with its hepar. Methods 72, mice , old age male ,on C57BL/6 background, were randomly divided into 3 groups(24/group): sham operation (group S);hepar’s partial resection （group H ）；hepar’s partial resection ＋celastrol （ group C ，60ug/kg/d ）。After being treated ， 8/group mice were arranged into water maze to be tested respectively,then mice’s ethology parameter were recorded.Also,the another 48 mice were performed to observe the expression of GFAP 、 CD68 protein in glial cells of hippocampus by immunohistochemistry methods. Results 1 the mice’s ethology parameter tested in the water maze indicated that retention and cognition were improved in 8 mice of group C at day 3-5 after mice’s being treated when compared with 8 mice of group H （P ＜0.05 ）；2 After being treated, the number of GFAP ＋ and CD68 ＋ glial cells in mice’s hippocampus trended to increase at 12h,and got to the peak at day 3,then trended to decrease till disappearing in 12 mice of group H,correspondingly, the number of GFAP＋ and CD68＋ glial cells in 12 mice of group C were always lower than those in group H through immunohistochemistry me