杭州湾水环境中氨氧化微生物对外源氨氮的响应-北京大学学报自然.pdfVIP

北京大学学报 ( 自然科学版), 第 49 卷 , 第 5 期 , 2013 年 9 月 Acta Scientiarum Naturalium Universitatis Pekinensis, Vol. 49, No. 5 (Sept. 2013) 杭州湾水环境中氨氧化微生物对外源氨氮的响应 孙仁华 温东辉† 北京大学环境科学与工程学院 , 北京 100871; † 通信作者 , E-mail: dhwen@ 摘要 采集杭州湾邻近工业园区纳污水域靠近排污点(A 点)及远离排污点(B 点) 的沉积物和水样, 进行实验室 模拟培养实验。在外源氨氮的刺激下 , 培养体系中微生物氨氧化作用加强, 35 天后水中投加的 NH4 +-N 全部 被去除, 而 NO3−-N 出现积累。源于 A, B 点的样品在培养实验后, 基于细菌氨单加氧酶基因(Bact-amoA) 的 DGGE 指纹图谱中的条带数由 1 条分别增加至 7 条和 5 条, 表明氨氧化细菌的群落结构发生变化, 多样性增 加 ; 而基于古菌氨单加氧酶基因(Arch-amoA) 的DGGE 条带数没有明显变化, 表明氨氧化古菌的群落结构没有 显著变化。 关键词 氨氧化细菌; 氨氧化古菌; 氨氧化; 外源氨氮 中图分类号 X172 Responses to External Ammonium Source by the Ammonia Oxidation Microorganisms from Hangzhou Bay SUN Renhua, WEN Donghui† College of Environmental Sciences and Engineering, Peking University, Beijing 100871; † Corresponding author, E-mail: dhwen@ Abstract A simulated cultivation was carried out in lab, using the sediment and seawater samples collected from the discharge-receiving sea near an industrial park in Hangzhou Bay. The samples were collected from two sites—one was near to the outlet (site A) and the other was far from the outlet (site B). Ammonia oxidation was stimulated and enhanced by the addition of ammonium in the sediment-seawater microsystems. After 35-day cultivation, all of the external NH + -N was removed, but NO− -N accumulated in the aqueous solution. The 4 3 PCR-DGGE det