conserved rules govern genetic interaction degree across species守恒的规则管理跨物种遗传相互作用程度.pdfVIP

Koch et al. Genome Biology 2012, 13:R57 /2012/13/7/R57 RESEARCH Open Access Conserved rules govern genetic interaction degree across species 1 2,3 1,4 1 5 Elizabeth N Koch , Michael Costanzo , Jeremy Bellay , Raamesh Deshpande , Kate Chatfield-Reed , 5 6 2,3 2,3 1* Gordon Chua , Gennaro D’Urso , Brenda J Andrews , Charles Boone and Chad L Myers Abstract Background: Synthetic genetic interactions have recently been mapped on a genome scale in the budding yeast Saccharomyces cerevisiae, providing a functional view of the central processes of eukaryotic life. Currently, comprehensive genetic interaction networks have not been determined for other species, and we therefore sought to model conserved aspects of genetic interaction networks in order to enable the transfer of knowledge between species. Results: Using a combination of physiological and evolutionary properties of genes, we built models that successfully predicted the genetic interaction degree of S. cerevisiae genes. Importantly, a model trained on S. cerevisiae gene features and degree also accurately predicted interaction degree in the fission yeast Schizosaccharomyces pombe, suggesting that many of the predictive relationships discovered in S. cerevisiae also hold in this evolutionarily distant yeast. In both species, high single mutant fitness defect, protein disorder, pleiotropy, protein-protein interaction network degree, and low expression variation were significantly predictive of genetic interaction degree. A comparison of the predicted genetic interaction degrees of S. pombe genes to the degre