芦荟多糖对体外培养人成纤维细胞增殖及胶原合成及分泌影响.pdf

both and InfluenceoftheAloevera on collagen polysaccharideproliferation andsecretion humanfibroblastcellsinvitro synthesis cultured Graduate：Liu Lingying Wu Supervisor-Boyu Chen Xiaodong Abstract objective： To theeffectoftheAloevera onthe and investigate polysaccharidesproliferation andsecretionofhumanfibroblastcellsculturedinvitro． collagensynthesis Method： 1．The were dividedinto fibroblastcellsculturedinvitro randomly control cell wasmeasured 50，100，200，400mg／L)andgroups．Theproliferation by MTT．Thecell Wasdetectedflow cell Was cycle by cytometryanalysis．Theapoptosis fortheevaluationtoxicsideeffectoftheAloevera AO-EB by assayed polysaccharides stain rateoflactate forthe method．The leakage assayed dehydrogenase(LDH)Was evaluationofthecell injury． mRNA 2．ThefibroblastcellsDNA wasmeasured of synthesis by【jHI-proline；Express intheculturedfibroblastWasmeasuredRT—PCR； I,III by collagentype