a novel cold-adapted lipase from sorangium cellulosum strain so0157-2 gene cloning, expression, and enzymatic characterization一种新型低温脂肪酶从sorangium cellulosum应变so0157-2基因克隆,表达和酶的特性.pdfVIP

下载本文档

8
0
约6.03万字
约 16页
2017-09-07 发布于上海
举报
版权申诉

a novel cold-adapted lipase from sorangium cellulosum strain so0157-2 gene cloning, expression, and enzymatic characterization一种新型低温脂肪酶从sorangium cellulosum应变so0157-2基因克隆,表达和酶的特性.pdf

1、本文档共16页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

a novel cold-adapted lipase from sorangium cellulosum strain so0157-2 gene cloning, expression, and enzymatic characterization一种新型低温脂肪酶从sorangium cellulosum应变so0157-2基因克隆,表达和酶的特性

Int. J. Mol. Sci. 2011, 12, 6765-6780; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article A Novel Cold-Adapted Lipase from Sorangium cellulosum Strain So0157-2: Gene Cloning, Expression, and Enzymatic Characterization Yuan-Yuan Cheng †, Yun-Kai Qian †, Zhi-Feng Li, Zhi-Hong Wu, Hong Liu and Yue-Zhong Li * State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, China; E-Mails: cyysdu@ (Y.-Y.C.); qyk-1@163.com (Y.-K.Q.); lizhifeng@ (Z.-F.L.); wuzhihong@ (Z.-H.W.); liuhong05@ (H.L.) † These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: lilab@; Tel./Fax: +86-531 Received: 23 August 2011; in revised form: 24 September 2011 / Accepted: 8 October 2011 / Published: 13 October 2011 Abstract: Genome sequencing of cellulolytic myxobacterium Sorangium cellulosum reveals many open-reading frames (ORFs) encoding various degradation enzymes with low sequence similarity to those reported, but none of them has been characterized. In this paper, a predicted lipase gene (lipA) was cloned from S. cellulosum strain So0157-2 and characterized. lipA is 981-bp in size, encoding a polypeptide of 326 amino acids that contains the pentapeptide (GHSMG) and catalytic triad residues (Ser114, Asp250 and His284). Searc