aconitase b is required for optimal growth of xanthomonas campestris pv. vesicatoria in pepper plants顺乌头酸酶b的最佳生长需要、黄定pv。.pdfVIP

Aconitase B Is Required for Optimal Growth of Xanthomonas campestris pv. vesicatoria in Pepper Plants 1 ¨ 2 1 1 Janine Kirchberg , Daniela Buttner , Barbara Thiemer , R. Gary Sawers * 1 Department of Microbiology, Institute of Biology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany, 2 Department of Genetics, Institute of Biology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany Abstract The aerobic plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria (Xcv) colonizes the intercellular spaces of pepper and tomato. One enzyme that might contribute to the successful proliferation of Xcv in the host is the iron-sulfur protein aconitase, which catalyzes the conversion of citrate to isocitrate in the tricarboxylic acid (TCA) cycle and might also sense reactive oxygen species (ROS) and changes in cellular iron levels. Xcv contains three putative aconitases, two of which, acnA and acnB, are encoded by a single chromosomal locus. The focus of this study is aconitase B (AcnB). acnB is co- transcribed with two genes, XCV1925 and XCV1926, encoding putative nucleic acid-binding proteins. In vitro growth of acnB mutants was like wild type, whereas in planta growth and symptom formation in pepper plants were impaired. While acnA, XCV1925 or XCV1926 mutants showed a wild-type phenotype with respect to bacterial growth and in planta symptom formation, proliferation of the acnB mutant in susceptible pepper plants was significantly impaired. Furthermore, the deletion of acnB led to reduced HR induction in resistant pepper plants and an increased susceptibility to the superoxide- generating compound menadione. As AcnB complemented the growth deficiency of an Escherichia c