aeromonas surface glucan attached through the o-antigen ligase represents a new way to obtain udp-glucose气单胞菌属表面葡聚糖通过o抗原连接酶连接获得udp-glucose代表了一个新的方法.pdfVIP

Aeromonas Surface Glucan Attached through the O- Antigen Ligase Represents a New Way to Obtain UDP- Glucose 1 1 2 2 ´ 3 Susana Merino , Lamiaa Bouamama , Yuriy A. Knirel , Sofya N. Senchenkova , Miguel Regue , ´ 1* Juan M. Tomas ´ ´ 1 Departamento de Microbiologıa, Facultad de Biologıa, Universidad de Barcelona, Diagonal, Barcelona, Spain, 2 N. D. Zelinsky Institute of Organic Chemistry, Russian ´ ´ Academy of Sciences, Moscow, Russia, 3 Departamento de Microbiologıa y Parasitologıa Sanitarias, Facultad de Farmacia, Universidad de Barcelona, Barcelona, Spain Abstract We previously reported that A. hydrophila GalU mutants were still able to produce UDP-glucose introduced as a glucose residue in their lipopolysaccharide core. In this study, we found the unique origin of this UDP-glucose from a branched a- glucan surface polysaccharide. This glucan, surface attached through the O-antigen ligase (WaaL), is common to the mesophilic Aeromonas strains tested. The Aeromonas glucan is produced by the action of the glycogen synthase (GlgA) and the UDP-Glc pyrophosphorylase (GlgC), the latter wrongly indicated as an ADP-Glc pyrophosphorylase in the Aeromonas genomes available. The Aeromonas glycogen synthase is able to react with UDP or ADP-glucose, which is not the case of E. coli glycogen synthase only reacting with ADP-glucose. The Aeromonas surface glucan has a role enhancing biofilm formation. Finally, for the first time to our knowledge, a clear preference on behalf of bacterial survival and pathogenesis is observed when choosing