afm-detected apoptotic changes in morphology and biophysical property caused by paclitaxel in ishikawa and hela cellsafm-detected凋亡形态学和生物物理属性的变化引起的紫杉醇在石川和海拉细胞.pdfVIP

AFM-Detected Apoptotic Changes in Morphology and Biophysical Property Caused by Paclitaxel in Ishikawa and HeLa Cells 1,2. 3. 4 5 3 Kyung Sook Kim , Chang Hoon Cho , Eun Kuk Park , Min-Hyung Jung , Kyung-Sik Yoon , Hun-Kuk Park1,2,6* 1 Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul, Korea, 2 Healthcare Industry Research Institute, Kyung Hee University, Seoul, Korea, 3 Department of Biochemistry and Molecular Biology, College of Medicine, Kyung Hee University, Seoul, Korea, 4 Department of Medical Zoology, College of Medicine, Kyung Hee University, Seoul, Korea, 5 Division of Gynecologic Oncology, College of Medicine, Kyung Hee Medical Center, Kyung Hee University, Seoul, Korea, 6 Program of Medical Engineering, Kyung Hee University, Seoul, Korea Abstract The apoptosis of cancer cells is associated with changes in the important cell properties including morphology, surface roughness and stiffness. Therefore, the changes in morphology and biophysical properties can be a good way of evaluating the anticancer activity of a drug. This study examined the effect of paclitaxel on the properties of Ishikawa and HeLa cells using atomic force microscopy (AFM), and the relationship between the changes in morphology and the biophysical properties and apoptosis was discussed. The viability and proliferation of the cells were analyzed using the methylthiazol tetrazolium (MTT) method and a TUNEL assay to confirm cellular apoptosis due to a paclitaxel treatment. AFM observations clearly showed the apoptotic morphological and biophysical changes in Ishikawa and HeLa cells. After the paclitaxel treatment, the cell membrane was torn and holed, the surface