图1KB对EC刺激RAW264.7细胞释放TNF-α和IL-6的抑制作用.docVIP

苦柯胺B对热刺激RAW 264.7细胞释放TNF-α和IL-6的热对热刺激RAW 264.7细胞释放TNF-α和IL-6Anti sepsis effects of kukoamine B in vitro and in vivo Yang Jingcheng1, Liu Xin1, Zheng Xinchuan1，Zhu Yuanfeng1, Lu Yongling1, Zheng Jiang1, Luo Qizhi2 1、400038，Medical Research Center, Southwestern Hospital, Third Military Medical University, Chongqing, China. 2、400038，Institute of Burn Research, State Key Lab of Burn, Truma, And Complication ,Chongqing, China. Objectives: to observe in vitro and in vivo the anti sepsis effects of kukoamine B (KB), an active component obtained from traditional Chinese medicine, Methods: heated killed bacteria liquids of Escherichia coli and Staphylococcus aureus were produced. The inhibitory effects of KB on TNF-α and IL-6 production in RAW 264.7 cells induced by the bacteria liquids were detected in vitro. In vivo, Mice sepsis models with graded severity were established by injection of different amounts of bacteria. Balb/c mice were challenged with EC or SA alone or in combination with KB（low, medium and high dosages）or anti sepsis drugs used currently in clinic (Xuebijing and Ulinastatin) separately. Mortality of mice in each group was observed for 7 days and mutual comparison was made between each group. Results: KB significantly attenuates the production of TNF-α and IL-6 in RAW 264.7 cells in a well dose dependent manner. KB also exhibits protective effects on sepsis model mice, as the survival of model mice in KB group is significantly higher than that in model group and anti sepsis drugs groups (p0.05 or p0.01). Conclusions: As a neutralizer for poly pathogen molecules，KB exhibits inhibitory effects of release of inflammatory cytokines. In addition, it has satisfactory protective effects on sepsis model animals. Such effects significantly outweigh that of Xuebijing and Ulinastatin, two anti sepsis drugs used currently in clinic. These results suggest that it is promising for KB to be developed as a novel anti sepsis drug candidate. Key words: Kukoamine B, sepsis,