quantitative field testing rotylenchulus reniformis dna from metagenomic samples isolated directly from soil定量实地测试rotylenchulus reniformis dna直接从土壤宏基因组样本孤立.pdfVIP

Quantitative Field Testing Rotylenchulus reniformis DNA from Metagenomic Samples Isolated Directly from Soil 1 1 1 2 3 Kurt Showmaker , Gary W. Lawrence , Shien Lu , Clarissa Balbalian , Vincent P. Klink * 1 Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, Mississippi, United States of America, 2 Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Bost Extension Center, Mississippi State University, Mississippi State, Mississippi, United States of America, 3 Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi, United States of America Abstract A quantitative PCR procedure targeting the b-tubulin gene determined the number of Rotylenchulus reniformis Linford Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence of other soil- dwelling plant parasitic nematodes including its sister genus Helicotylenchus Steiner, 1945. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from soil. Citation: Showmaker K, Lawrence GW, Lu S, Balbalian C, Klink VP (2011) Quantitative Field Testing Rotylenchulus reniformis DNA from Metagenomic Samples Isolated Directly from Soil. PLoS ONE 6(12): e28954. doi:10.1371/journal.pone.0028954 Editor: Zhanjiang Liu, Auburn University, United States of America Received September 26, 2011; Accepted November 17, 2011; Published December 16, 2011 Copyright: 2011 Showmaker et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the or