quantitative proteomics of spodoptera frugiperda cells during growth and baculovirus infection定量蛋白质组学spodoptera frugiperda细胞在生长和杆状病毒感染.pdfVIP

Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection 1 2 2 ´ 1,3 2 Nuno Carinhas , Aaron Mark Robitaille , Suzette Moes , Manuel Jose Teixeira Carrondo , Paul Jenoe , 4 1 Rui Oliveira , Paula Marques Alves * ´ ´ ´ 1 Instituto de Tecnologia Quımica e Biologica-Universidade Nova de Lisboa/Instituto de Biologia Experimental e Tecnologica, Oeiras, Portugal, 2 Department of ´ ˆ Biochemistry, Biozentrum of the University of Basel, Basel, Switzerland, 3 Departamento de Quımica, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, ´ ˆ Caparica, Portugal, 4 REQUIMTE, Systems Biology and Engineering Group (SBE), Departamento de Quımica, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal Abstract Baculovirus infection of Spodoptera frugiperda cells is a system of choice to produce a range of recombinant proteins, vaccines and, potentially, gene therapy vectors. While baculovirus genomes are well characterized, the genome of S. frugiperda is not sequenced and the virus-host molecular interplay is sparsely known. Herein, we describe the application of stable isotope labeling by amino acids in cell culture (SILAC) to obtain the first comparative proteome