quaternary structure heterogeneity of oligomeric proteins a saxs and sans study of the dissociation products of octopus vulgaris hemocyanin寡聚蛋白质的四级结构异质性的粉煤灰和无研究离解的产品章鱼寻常的血蓝蛋白.pdfVIP

Quaternary Structure Heterogeneity of Oligomeric Proteins: A SAXS and SANS Study of the Dissociation Products of Octopus vulgaris Hemocyanin 1 1 ˇ ´2 3 3 Francesco Spinozzi *, Paolo Mariani , Ivan Micetic , Claudio Ferrero , Diego Pontoni , Mariano Beltramini2* 1 Department of Life and Environmental Sciences, Marche Polytechnic University and CNISM, Ancona, Italy, 2 Department of Biology, University of Padova, Padova, Italy, 3 European Synchrotron Radiation Facility, Grenoble, France Abstract Octopus vulgaris hemocyanin shows a particular self-assembling pattern, characterized by a hierarchical organization of monomers. The highest molecular weight aggregate is a decamer, the stability of which in solution depends on several parameters. Different pH values, buffer compositions, H O/D O ratios and Hofmeister’s salts result in modifications of the 2 2 aggregation state of Octopus vulgaris hemocyanin. The new QUAFIT method, recently applied to derive the structure of the decameric and the monomeric assembly from small-angle scattering data, is used here to model the polydisperse system that results from changing the solution conditions. A dataset of small-angle X-rays and neutron scattering curves is analysed by QUAFIT to derive structure, composition and concentration of different assemblies present in solution. According to the hierarchy of the association/dissociation processes and the possible number of different aggregation products in solution, each sample has been considered as a heterogeneous mixture composed of the entire decamer, the dissociated ‘‘loose’’ monomer and all the intermediate