quantitative x-ray tomography of the mouse cochlea小鼠耳蜗的定量x射线断层扫描.pdfVIP

Quantitative X-ray Tomography of the Mouse Cochlea 1,2 2 3 2,4,5 Christoph Rau , Margaret Hwang , Wah-Keat Lee , Claus-Peter Richter * 1 Diamond Light Source Ltd., Diamond House, Harwell Science and Innovation Campus, Didcot, United Kingdom, 2 Department of Otolaryngology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America, 3 Advanced Photon Source, Argonne National Laboratory, Argonne, Illinois, United States of America, 4 Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States of America, 5 The Hugh Knowles Center, Department of Communication Sciences and Disorders, Northwestern University, Evanston, Illinois, United States of America Abstract Imaging with hard X-rays allows visualizing cochlear structures while maintaining intrinsic qualities of the tissue, including structure and size. With coherent X-rays, soft tissues, including membranes, can be imaged as well as cells making use of the so-called in-line phase contrast. In the present experiments, partially coherent synchrotron radiation has been used for micro-tomography. Three-dimensional reconstructions of the mouse cochlea have been created using the EM3D software and the volume has been segmented in the Amira Software Suite. The structures that have been reconstructed include scala tympani, scala media, scala vestibuli, Reissner’s membrane, basilar membrane, tectorial membrane, organ of Corti, spiral limbus, spiral ganglion and cochlear nerve. Cross-sectional areas of the scalae were measured. The results provide a realistic and quantitative reconstruction of the cochlea. Citation: Rau C, Hwang M, Lee W-K, Richter C-P (2012) Quantitative X-ray