quorum sensing influences vibrio harveyi growth rates in a manner not fully accounted for by the marker effect of bioluminescence鳗弧菌群体感应影响增长率的方式不能完全占生物荧光标记的效果的.pdfVIP

Quorum Sensing Influences Vibrio harveyi Growth Rates in a Manner Not Fully Accounted For by the Marker Effect of Bioluminescence 1 2 3 1 1 Zeena E. Nackerdien , Alexander Keynan , Bonnie L. Bassler , Joshua Lederberg , David S. Thaler * 1 Raymond and Beverly Sackler Laboratory of Molecular Genetics and Informatics, Rockefeller University, New York, New York, United States of America, 2 Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel, 3 Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, Princeton, New Jersey, United States of America Abstract Background: The light-emitting Vibrios provide excellent material for studying the interaction of cellular communication with growth rate because bioluminescence is a convenient marker for quorum sensing. However, the use of bioluminescence as a marker is complicated because bioluminescence itself may affect growth rate, e.g. by diverting energy. Methodology/Principal Findings: The marker effect was explored via growth rate studies in isogenic Vibrio harveyi (Vh) strains altered in quorum sensing on the one hand, and bioluminescence on the other. By hypothesis, growth rate is energy limited: mutants deficient in quorum sensing grow faster because wild type quorum sensing unleashes bioluminescence and bioluminescence diverts energy. Findings reported here confirm a role for bioluminescence in limiting Vh growth rate, at least under the conditions tested. However, the results argue that the bioluminescence is insufficient to explain the relationship of growth rate and quorum sensing in Vh. A Vh mutant null for all genes encoding the bioluminescence