rag1？？ mutant zebrafish demonstrate specific protection following bacterial re-exposurerag1 突变斑马鱼演示特定保护后细菌再次接触.pdfVIP

Rag12/ 2 Mutant Zebrafish Demonstrate Specific Protection following Bacterial Re-Exposure Claudia Hohn, Lora Petrie-Hanson* Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Starkville, Mississippi, United States of America Abstract Background: Recombination activation gene 1 deficient (rag12/ 2) mutant zebrafish have a reduced lymphocyte-like cell population that lacks functional B and T lymphocytes of the acquired immune system, but includes Natural Killer (NK)-like cells and Non-specific cytotoxic cells (NCC) of the innate immune system. The innate immune system is thought to lack the adaptive characteristics of an acquired immune system that provide enhanced protection to a second exposure of the same pathogen. It has been shown that NK cells have the ability to mediate adaptive immunity to chemical haptens and cytomegalovirus in murine models. In this study we evaluated the ability of rag12/ 2 mutant zebrafish to mount a protective response to the facultative intracellular fish bacterium Edwardsiella ictaluri. Methodology/Principal Findings: Following secondary challenge with a lethal dose of homologous bacteria 4 and 8 weeks after a primary vaccination, rag12/ 2 mutant zebrafish demonstrated protective immunity. Heterologous bacterial exposures did not provide protection. Adoptive leukocyte transfers from previously exposed mutants conferred protective immunity ¨ to naıve mutants when exposed to homologous bacteria. Conclusions/Significance: Our findings show that a component of the innate immune system mounted a response that provided significantly increased survival when rag12/ 2 mutant zebrafish were re-exposed to the same bacteria. Further, adoptive cell transfers demonstrated that kidney interstitial leukocytes from previously exposed rag12/ 2 mutant zebrafi