rapid selection and proliferation of cd133(+) cells from cancer cell lines chemotherapeutic implications快速选择和扩散cd133(+)细胞癌症细胞系化疗的影响.pdfVIP

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rapid selection and proliferation of cd133(+) cells from cancer cell lines chemotherapeutic implications快速选择和扩散cd133(+)细胞癌症细胞系化疗的影响.pdf

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rapidselectionandproliferationofcd133()cellsfromcancercelllineschemotherapeuticimplications快速选择和扩散cd133()细胞癌症细胞系化疗的影响

Rapid Selection and Proliferation of CD133(+) Cells from Cancer Cell Lines: Chemotherapeutic Implications 1 1,2 1,3,4 1 1 Sarah E. Kelly , Altomare Di Benedetto , Adelaide Greco , Candace M. Howard , Vincent E. Sollars , 1 5 1,6 Donald A. Primerano , Jagan V. Valluri , Pier Paolo Claudio * 1 Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine, Marshall University, Huntington, West Virginia, United States of America, 2 Department of Basic and Applied Biology, Faculty of Sciences, University of L’Aquila, L’Aquila, Italy, 3 CEINGE-Advanced Biotechnology, s.c.ar.l., Naples, Italy, 4 Department of Biomorphological and Functional Science, University of Naples ‘‘Federico II’’, and IBB-CNR, Naples, Italy, 5 Department of Biology, Marshall University, Huntington, West Virginia, United States of America, 6 Department of Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, West Virginia, United States of America Abstract Cancer stem cells (CSCs) are considered a subset of the bulk tumor responsible for initiating and maintaining the disease. Several surface cellular markers have been recently used to identify CSCs. Among those is CD133, which is expressed by hematopoietic progenitor cells as well as embryonic stem cells and various cancers. We have recently isolated and cultured CD133 positive [CD133(+)] cells from various cancer cell lines using a NASA developed Hydrodynamic Focusing Bioreactor (HFB) (Celdyne, Houston, TX). For comparison, another bioreactor, the rotary cell culture system (RCCS) manufactured by Synthecon (Houston, TX)