reconstitution of the costunolide biosynthetic pathway in yeast and nicotiana benthamiana调整的costunolide酵母生物合成途径和烟草benthamiana.pdfVIP

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reconstitution of the costunolide biosynthetic pathway in yeast and nicotiana benthamiana调整的costunolide酵母生物合成途径和烟草benthamiana.pdf

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reconstitution of the costunolide biosynthetic pathway in yeast and nicotiana benthamiana调整的costunolide酵母生物合成途径和烟草benthamiana

Reconstitution of the Costunolide Biosynthetic Pathway in Yeast and Nicotiana benthamiana 1. 2. 1,3 1 1 Qing Liu , Mohammad Majdi , Katarina Cankar , Miriam Goedbloed , Tatsiana Charnikhova , 1 3,4,5 1,3 1 Francel W. A. Verstappen , Ric C. H. de Vos , Jules Beekwilder , Sander van der Krol , Harro J. Bouwmeester1* 1 Laboratory of Plant Physiology, Wageningen University, Wageningen, The Netherlands, 2 Agronomy and Plant Breeding Department, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran, 3 Plant Research International, Wageningen, The Netherlands, 4 Centre for BioSystems Genomics, Wageningen, The Netherlands, 5 Netherlands Metabolomics Centre, Leiden, The Netherlands Abstract The sesquiterpene costunolide has a broad range of biological activities and is the parent compound for many other biologically active sesquiterpenes such as parthenolide. Two enzymes of the pathway leading to costunolide have been previously characterized: germacrene A synthase (GAS) and germacrene A oxidase (GAO), which together catalyse the biosynthesis of germacra-1(10),4,11(13)-trien-12-oic acid. However, the gene responsible for the last step toward costunolide has not been characterized until now. Here we show that chicory costunolide synthase (CiCOS), CYP71BL3, can catalyse the oxidation of germacra-1(10),4,11(13)-trien-12-oic acid to yield costunolide. Co-expression of feverfew GAS (TpGAS), chicory GAO (CiGAO), and chicory COS (CiCOS) in yeast resulted in the biosynt