recruitment of polo kinase to the spindle midzone during cytokinesis requires the feoklp3a complex招聘的马球激酶主轴midzone胞质分裂过程中需要feoklp3a复杂.pdfVIP

Recruitment of Polo Kinase to the Spindle Midzone during Cytokinesis Requires the Feo/Klp3A Complex 1 1 1 2 2 2 1 Pier Paolo D’Avino *, Vincent Archambault , Marcin R. Przewloka , Wei Zhang , Kathryn S. Lilley , Ernest Laue , David M. Glover 1 Cancer Research UK Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Cambridge, United Kingdom, 2 Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom Background. Polo-like kinases control multiple events during cell division, including mitotic entry, centrosome organization, spindle formation, chromosome segregation and cytokinesis. Their roles during cytokinesis, however, are not well understood because the requirement of these kinases during early stages of mitosis complicates the study of their functions after anaphase onset. Methodology/Principal Findings. We used time-lapse microscopy to analyze the dynamics of Polo::GFP in Drosophila tissue culture cells during mitosis. After anaphase onset, Polo::GFP concentrated at the spindle midzone, but also diffused along the entire length of the central spindle. Using RNA interference we demonstrate that the microtubule- associated proteins Feo and Klp3A are required for Polo recruitment to the spindle midzone, but not the kinesin Pavarotti as previously thought. Moreover, we show that Feo and Klp3A form a complex and that Polo co-localizes with both proteins during cytokinesis. Conclusion/Significance. Our results reveal that the Feo/Klp3A complex is necessary for Polo recruitment to the spindle midzone. A similar finding has also been recently reported in mammalian cells [1], suggesting that this basic mechanism has been conserved during evolution, albeit with