regulatory t cells enhance susceptibility to experimental trypanosoma congolense infection independent of mouse genetic background调节性t细胞增强敏感性实验锥虫属congolense感染小鼠遗传背景独立的.pdfVIP

Regulatory T Cells Enhance Susceptibility to Experimental Trypanosoma Congolense Infection Independent of Mouse Genetic Background 1 2 2 2 2 1,2 Ifeoma Okwor , Chukwunonso Onyilagha , Shiby Kuriakose , Zhirong Mou , Ping Jia , Jude E. Uzonna * 1 Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada, 2 Department of Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada Abstract Background: BALB/c mice are highly susceptible while C57BL/6 are relatively resistant to experimental Trypanosoma congolense infection. Although regulatory T cells (Tregs) have been shown to regulate the pathogenesis of experimental T. congolense infection, their exact role remains controversial. We wished to determine whether Tregs contribute to distinct phenotypic outcomes in BALB/c and C57BL/6 mice and if so how they operate with respect to control of parasitemia and production of disease-exacerbating proinflammatory cytokines. Methodology/Findings: BALB/c and C57BL/6 mice were infected intraperitoneally (i.p) with 103 T. congolense clone TC13 and both the kinetics of Tregs expansion and intracellular cytokine profiles in the spleens and livers were monitored directly ex vivo by flow cytometry. In some experiments, mice were injected with anti-CD25 mAb prior or post T. congolense ¨ + infection or adoptively (by intravenous route) given highly enriched naıve CD25 T lymphocytes prior to T. congolense infection and the inflammatory c