rna aptamers generated against oligomeric aβ40 recognize common amyloid aptatopes with low specificity but high sensitivityrna寡核苷酸适配子与低聚物的生成aβ40识别常见的淀粉样蛋白aptatopes特异性较低,但灵敏度高.pdfVIP

RNA Aptamers Generated against Oligomeric Ab40 Recognize Common Amyloid Aptatopes with Low Specificity but High Sensitivity 1 1¤ 1 2,4 1,3,4 Farid Rahimi , Kazuma Murakami , Jamie L. Summers , Chi-Hong B. Chen , Gal Bitan * 1 Department of Neurology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America, 2 Department of Biological Chemistry, University of California Los Angeles, Los Angeles, California, United States of America, 3 Brain Research Institute, University of California Los Angeles, Los Angeles, California, United States of America, 4 Molecular Biology Institute, University of California Los Angeles, Los Angeles, California, United States of America Abstract Aptamers are useful molecular recognition tools in research, diagnostics, and therapy. Despite promising results in other fields, aptamer use has remained scarce in amyloid research, including Alzheimer’s disease (AD). AD is a progressive neurodegenerative disease believed to be caused by neurotoxic amyloid b-protein (Ab) oligomers. A b oligomers therefore are an attractive target for development of diagnostic and therapeutic reagents. We used covalently-stabilized oligomers of the 40-residue form of A b (Ab40) for aptamer selection. Despite gradually increasing the stringency of selection conditions, the selected aptamers did not recognize A b40 oligomers but reacted with fibrils of A b40, A b42, and several other amyloidogenic proteins. Aptamer reactivity with amyloid fibrils showed some degree of protein-sequence dependency.