root suberin forms an extracellular barrier that affects water relations and mineral nutrition in arabidopsis根软木脂细胞外形成了一个屏障,影响水的关系在拟南芥和矿物质营养.pdfVIP

Root Suberin Forms an Extracellular Barrier That Affects Water Relations and Mineral Nutrition in Arabidopsis 1 2 2 2 3 Ivan Baxter , Prashant S. Hosmani , Ana Rus , Brett Lahner , Justin O. Borevitz , Balasubramaniam 2 2 4 4 1,2 Muthukumar , Michael V. Mickelbart , Lukas Schreiber , Rochus B. Franke , David E. Salt * 1 Bindley Bioscience Center, Purdue University, West Lafayette, Indiana, United States of America, 2 Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana, United States of America, 3 Department of Ecology and Evolution, University of Chicago, Chicago, Illinois, United States of America, 4 Institute of Cellular and Molecular Botany, University of Bonn, Bonn, Germany Abstract Though central to our understanding of how roots perform their vital function of scavenging water and solutes from the soil, no direct genetic evidence currently exists to support the foundational model that suberin acts to form a chemical barrier limiting the extracellular, or apoplastic, transport of water and solutes in plant roots. Using the newly characterized enhanced suberin1 (esb1) mutant, we established a connection in Arabidopsis thaliana between suberin in the root and both water movement through the plant and solute accumulation in the shoot. Esb1 mutants, characterized by increased root suberin, were found to have reduced day time transpiration rates and increased water-use efficiency during their vegetative growth period. Furthermore, these changes in suberin and water transport were associated with decreases