rubber elongation factor (ref), a major allergen component in hevea brasiliensis latex has amyloid properties橡胶延长因子(ref),主要的过敏原组件乳胶橡胶树割面干涸病淀粉样属性.pdfVIP

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rubber elongation factor (ref), a major allergen component in hevea brasiliensis latex has amyloid properties橡胶延长因子(ref),主要的过敏原组件乳胶橡胶树割面干涸病淀粉样属性.pdf

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rubber elongation factor (ref), a major allergen component in hevea brasiliensis latex has amyloid properties橡胶延长因子(ref),主要的过敏原组件乳胶橡胶树割面干涸病淀粉样属性

Rubber Elongation Factor (REF), a Major Allergen Component in Hevea brasiliensis Latex Has Amyloid Properties 1 2 1 ´ ´ 3 4 Karine Berthelot *, Sophie Lecomte , Yannick Estevez , Benedicte Coulary-Salin , Ahmed Bentaleb , 3 1 ´ ´ 1 Christophe Cullin , Alain Deffieux , Frederic Peruch * 1 CNRS, LCPO, UMR 5629, Pessac, France, 2 CNRS, CBMN, UMR 5248, Pessac, France, 3 Univ. Bordeaux 2, CNRS, IBGC, UMR 5095, Bordeaux, France, 4 CNRS, CRPP, UPR 8641, Pessac, France Abstract REF (Hevb1) and SRPP (Hevb3) are two major components of Hevea brasiliensis latex, well known for their allergenic properties. They are obviously taking part in the biosynthesis of natural rubber, but their exact function is still unclear. They could be involved in defense/stress mechanisms after tapping or directly acting on the isoprenoid biosynthetic pathway. The structure of these two proteins is still not described. In this work, it was discovered that REF has amyloid properties, contrary to SRPP. We investigated their structure by CD, TEM, ATR-FTIR and WAXS and neatly showed the presence of b- sheet organized aggregates for REF, whereas SRPP mainly fold as a helical protein. Both proteins are highly hydrophobic but differ in their interaction with lipid monolayers used to mimic the monomembrane surrounding the rubber particles. Ellipsometry experiments showed that REF seems to penetrate deeply into the monolayer and SRPP only binds to the lipid surface. These results could therefore clar