safety and immunogenicity of h5n1 influenza vaccine based on baculovirus surface display system of bombyx morih5n1流感疫苗的安全性和免疫原性家蚕杆状病毒表面展示系统的基础上.pdfVIP

Safety and Immunogenicity of H5N1 Influenza Vaccine Based on Baculovirus Surface Display System of Bombyx mori 1,2. 2. 2. 2 2 2 2 Rongzhong Jin , Zhengbing Lv , Qin Chen , Yanping Quan , Haihua Zhang , Si Li , Guogang Chen , 2 2 2 3 1,2 Qingliang Zheng , Lairong Jin , Xiangfu Wu , Jianguo Chen *, Yaozhou Zhang * 1 College of Life Sciences, Zhejiang University, Hangzhou, China, 2 Institute of Biochemistry, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China, 3 College of Life Sciences, Peking University, Beijing, China Abstract Avian influenza virus (H5N1) has caused serious infections in human beings. This virus has the potential to emerge as a pandemic threat in humans. Effective vaccines against H5N1 virus are needed. A recombinant Bombyx mori baculovirus, Bmg64HA, was constructed for the expression of HA protein of H5N1 influenza virus displaying on the viral envelope surface. The HA protein accounted for approximately 3% of the total viral proteins in silkworm pupae infected with the recombinant virus. Using a series of separation and purification methods, pure Bmgp64HA virus was isolated from these silkworm pupae bioreactors. Aluminum hydroxide adjuvant was used for an H5N1 influenza vaccine. Immunization with this vaccine at doses of 2 mg/kg and 0.67 mg/kg was carried out to induce the production of neutralizing antibodies, which protected monkeys against influenza virus infection. At these doses, the vaccine induced 1:40 antibody titers in 50% and 67% of the monkeys, respectively. The results of safet