segregating yku80 and tlc1 alleles underlying natural variation in telomere properties in wild yeast隔离yku80和tlc1等位基因潜在的自然变异在野生酵母端粒属性.pdfVIP

Segregating YKU80 and TLC1 Alleles Underlying Natural Variation in Telomere Properties in Wild Yeast 1 1,2 1 1 1 Gianni Liti *, Svasti Haricharan , Francisco A. Cubillos , Anna L. Tierney , Sarah Sharp , Alison A. 2 3 1 1 Bertuch , Leopold Parts , Elizabeth Bailes , Edward J. Louis * 1 Institute of Genetics, Queen’s Medical Centre, University of Nottingham, Nottingham, United Kingdom, 2 Baylor College of Medicine, Houston, Texas, United States of America, 3 The Wellcome Trust Sanger Institute, Hinxton, United Kingdom Abstract In yeast, as in humans, telomere length varies among individuals and is controlled by multiple loci. In a quest to define the extent of variation in telomere length, we screened 112 wild-type Saccharomyces sensu stricto isolates. We found extensive telomere length variation in S. paradoxus isolates. This phenotype correlated with their geographic origin: European strains were observed to have extremely short telomeres (,150 bp), whereas American isolates had telomeres approximately three times as long (.400 bp). Insertions of a URA3 gene near telomeres allowed accurate analysis of individual telomere lengths and telomere position effect (TPE). Crossing the American and European strains resulted in F1 spores with a continuum of telomere lengths consistent with what would be predicted if many quantitative trait loci (QTLs) were involved in length maintenance. Variation in TPE is similarly quantitative but only weakly correlated with telomere length. Genotyping F1 segregants indicated several QTLs associated with telomere length and silencing variation. These QTLs include likely candidate genes but also