selection of dna aptamers against glioblastoma cells with high affinity and specificity选择对胶质母细胞瘤细胞dna寡核苷酸适配子与高亲和力和特异性.pdfVIP

Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity 1 1 2 2 2 2 1 Dezhi Kang *, Jiangjie Wang , Weiyun Zhang , Yanling Song , Xilan Li , Yuan Zou , Mingtao Zhu , 2 1 2 Zhi Zhu , Fuyong Chen , Chaoyong James Yang * 1 Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China, 2 State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Science and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, China Abstract Background: Glioblastoma is the most common and most lethal form of brain tumor in human. Unfortunately, there is still no effective therapy to this fatal disease and the median survival is generally less than one year from the time of diagnosis. Discovery of ligands that can bind specifically to this type of tumor cells will be of great significance to develop early molecular imaging, targeted delivery and guided surgery methods to battle this type of brain tumor. Methodology/Principal Findings: We discovered two target-specific aptamers named GBM128 and GBM131 against cultured human glioblastoma cell line U118-MG after 30 rounds selection by a method called cell-based Systematic Evolution of Ligands by EXponential enrichment (cell-SELEX). These two aptamers have high affinity and specificity against target glioblastoma cells. They neither recognize normal astraglial cells, nor do they recognize other normal and cancer cel