setting of methods for analysis of mucosal antibodies in seminal and vaginal fluids of hiv seropositive subjects from cambodian and italian cohorts设置方法分析精液和阴道粘膜抗体的液体从柬埔寨和意大利军团艾滋病毒血清反应阳性的科目.pdfVIP
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setting of methods for analysis of mucosal antibodies in seminal and vaginal fluids of hiv seropositive subjects from cambodian and italian cohorts设置方法分析精液和阴道粘膜抗体的液体从柬埔寨和意大利军团艾滋病毒血清反应阳性的科目
Setting of Methods for Analysis of Mucosal Antibodies in
Seminal and Vaginal Fluids of HIV Seropositive Subjects
from Cambodian and Italian Cohorts
1.¤a 1.¤b 2 1 3¤c
Carla Donadoni , Cinzia Bisighini , Lorenza Scotti , Lorenzo Diomede , Marie Ngyen , Janin
3 4 2 5 6 2
Nouhin , Lucia DeSantis , Antonella Zambon , Davide Ferrari , Giulia Gallotta , Giovanni Corrao ,
7 1
Gianfranco Pancino , Lucia Lopalco *
1 Division of Immunology, Infectious Diseases and Trasplantation, San Raffaele Scientific Institute, Milan, Italy, 2 Department of Statistics, University of Milano-Bicocca,
Milan, Italy, 3 HIV/Hepatitis Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia, 4 Department of Obstetrics and Gynaecology, University Vita Salute, San
Raffaele Scientific Institute, Milan, Italy, 5 Department of Obstetrics and Gynaecology, San Raffaele Scientific Institute, Milan, Italy, 6 Department of Infectious Diseases, San
Raffaele Scientific Institute, Milan, Italy, 7 Regulation of Retroviral Infections Unit, Institut Pasteur, Paris, France
Abstract
Background: Genital mucosae play a key role in protection from STD and HIV infection, due to their involvement in both
horizontal and vertical disease transmission. High variability of published observations concerning IgA isolation and
quantification underlies the strong requirement of specific methods able to maximize investigation on HIV-specific IgA.
Methodology: Genital fluids from 109 subjects, including male and female cohorts from Italy and Cambodia, were co
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