shift of bacterial community in synanthropic mite tyrophagus putrescentiae induced by fusarium fungal diet转变的细菌社区synanthropic螨tyrophagus putrescentiae镰刀菌素真菌引起的饮食.pdfVIP

Shift of Bacterial Community in Synanthropic Mite Tyrophagus putrescentiae Induced by Fusarium Fungal Diet 1 ´ 1 ´ ´ ´ ˇ ´ 2 ´ 2 Jan Hubert , Marta Nesvorna , Marketa Sagova-Mareckova , Jan Kopecky * 1 Department of Pest Control of Stored Products and Food Safety, Crop Research Institute, Prague, Czech Republic, 2 Laboratory for Diagnostics and Epidemiology of Microorganisms, Crop Research Institute, Prague, Czech Republic Abstract Background: Tyrophagus putrescentiae (Acari: Astigmata) and Fusarium sp. co-occur in poorly managed grain. In a laboratory experiment, mite grazing resulted in significant reduction of fungal mycelium on cultivation plates. The destruction of mycelium appeared to be a result of an interaction between the mites, fungi and associated bacteria. Methodology and Principal Findings: A laboratory experiment was performed to simulate a situation of grain multiinfested by mites and Fusarium fungi. Changes of mite-associated bacterial community in T. putrescentiae were described in 3 habitats: (i) T. putrescentiae mites from a rearing diet prior to their transfer to fungal diet; (ii) fungal mycelium before mite introduction; (iii) mites after 7 day diet of each Fusarium avenaceum, F. culmorum, F. poae and F. verticillioides. Bacterial communities were characterized by 16 S rRNA gene sequencing. In total, 157 nearly full-length 16 S rRNA gene sequences from 9 samples representing selected habitats were analyzed. In the mites, the shift from rearing to fungal diet caused changes in mite associated bacterial community. A diverse ba