shikonin increases glucose uptake in skeletal muscle cells and improves plasma glucose levels in diabetic goto-kakizaki rats紫草素增加骨骼肌细胞中葡萄糖的吸收,改善糖尿病患者的血糖水平goto-kakizaki老鼠.pdfVIP

Shikonin Increases Glucose Uptake in Skeletal Muscle Cells and Improves Plasma Glucose Levels in Diabetic Goto-Kakizaki Rats ¨ 1. 2. 1 1 1 Anette I. Oberg , Kamal Yassin , Robert I. Csikasz , Nodi Dehvari , Irina G. Shabalina , Dana S. 3,4 5 ¨ ¨ 2 1 Hutchinson , Mona Wilcke , Claes-Goran Ostenson , Tore Bengtsson * 1 Department of Physiology, Arrhenius Laboratories F3, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden, 2 Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden, 3 Department of Pharmacology, Monash University, Parkville, Victoria, Australia, 4 Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria, Australia, 5 Glucox Biotech AB, Stockholm, Sweden Abstract Background: There is considerable interest in identifying compounds that can improve glucose homeostasis. Skeletal muscle, due to its large mass, is the principal organ for glucose disposal in the body and we have investigated here if shikonin, a naphthoquinone derived from the Chinese plant Lithospermum erythrorhizon, increases glucose uptake in skeletal muscle cells. Methodology/Principal Findings: Shikonin increases glucose uptake in L6 skeletal muscle myotubes, but does not phosphorylate Akt, indicating that in skeletal muscle cells its effect is medaited via a pathway distinct from that used for insulin-stimulated uptake. Furthermore we find no evidence for the involvement of AMP-activated protein kinase in shi