骨骼肌缺血后调适动态pH测量及模拟酸灌注减轻缺血再灌注损伤.docVIP

骨骼肌缺血后调适动态pH测量及模拟酸灌注减轻缺血再灌注损伤 阮思杰，阳富春，阳茂春，刘军廷，胡 峰，王静威(广西医科大学第一附属医院创伤骨科手外科，广西壮族自治区南宁市 530021) 引用本文：阮思杰，阳富春，阳茂春，刘军廷，胡峰，王静威. 骨骼肌缺血后调适动态pH测量及模拟酸灌注减轻缺血再灌注损伤[J].中国组织工程研究，2017，21():2558-2564. DOI:10.3969/j.issn.2095-4344.2017.16.017 ORCID: 0000-0003-1254-573X(阮思杰) 文章快速阅读： 文题释义： 缺血后调适：又名缺血后处理或缺血后适应，组织经受长时间缺血后，在再灌注前，对组织进行数个短暂的再灌/缺血循环的处理，可有效减轻其缺血再灌注损伤。 RISK通路：①缺血后调适于再灌注初期出现一个延长酸性平台，pH为6.81±0.133，时长为2 min 40 s。②乳酸脱氢酶、髓过氧化物酶、湿/干质量比值检测结果显示：缺血后调适组、乳酸酸灌注组明显低于缺血再灌注组(P 0.05)；③Western Blot 分析显示：p-Erk的表达缺血后调适组、乳酸酸灌注组及生理盐水组均显著高于缺血再灌注组(P 0.05)④TTC染色检测显示，缺血后调适组及乳酸酸灌注组梗死面积较缺血再灌注组明显减少(P 0.05)⑤结论：缺血后调适可维持再灌注初期短暂酸性状态；酸性灌注液可以有效模拟缺血后调适作用减少大鼠骨骼肌缺血再灌注损伤。其机制可能通过Erk1/2磷酸化激活RISK信号通路而起到保护作用。 Dynamic pH measurement in the skeletal muscle during ischemic postconditioning and simulated infusion with acidic perfusate to attenuate ischemia/reperfusion injury Ruan Si-jie, Yang Fu-chun, Yang Mao-chun, Liu Jun-ting, Hu Feng, Wang Jing-wei (Department of Trauma Orthopedics Hand Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China) Abstract BACKGROUND: Ischemic postconditioning protects the myocardium from ischemia/recursion injury via maintaining 3-minute acidosis initially. But its effect on the skeletal muscle remains unclear. OBJECTIVE: To dynamically measure the pH values in rat skeletal muscle after ischemia, and then to simulate acidic perfusate infusion to investigate the effect of ischemic postconditioning on ischemia/reperfusion injury. METHODS: Based on the ischemia/reperfusion injury model and ischemic postconditioning protocol in previous study, dynamic measurement of pH values in rat skeletal muscle was conducted using pH instrument at the global ischemia, ischemic postconditioning (30/30 seconds) and reperfusion period, and then the acidic perfusate equivalent to pH in ischemic postconditioning period was prepared with lactic acid and normal saline. Twenty-five healthy adult male Sprague-Dawley rats were ra