5-HT细胞移植对慢性不可预见性抑郁症大鼠多器官损伤作用及可能.docVIP

5-HT细胞移植对慢性不可预见性抑郁症大鼠多器官损伤作用及可能 　 作者：吴爱群　许金明　周强　姬瑞娟　党瑞山　吕宝来　王栋　陈尔瑜 【摘要】 目的 探讨外源性5-HT神经细胞移植对慢性不可预见性应激大鼠多内脏器官损伤的作用。方法 将40只雄性SD大鼠（购置第二军医大学实验动物中心）随机分为4组（10只/组）：正常对照组（Ⅰ）、慢性应激组（Ⅱ）、慢性应激细胞移植组（Ⅲ）、慢性应激氟西汀治疗组（Ⅳ）。采用慢性不可预见性应激抑郁模型法造模后，将5-HT神经细胞1次性移植于Ⅲ组大鼠的蛛网膜下腔，Ⅳ组每天给药1次3周；经Open-fie1d行为测试，解剖观察、取材、切片，HE和免疫组化法等染色，镜下观察。结果 （1）与Ⅱ组相比，Ⅲ和Ⅳ组大鼠行为明显改善；（2）大体解剖显示：Ⅱ组大鼠肺出现灶状充血，30％出现肺脓肿现象；Ⅲ组大鼠肺病变明显减轻，未见肺脓肿发生；（3）HE和免疫组化染色：Ⅱ组大鼠出现胃腺萎缩，肺组织间炎症细胞增多；Ⅲ和Ⅳ组大鼠胃腺和食管黏膜上皮萎缩以及肺组织炎症反应减轻，5-HT免疫反应增强，阳性细胞数目增多，与Ⅱ组相比差异有显著性（Plt;0.01）；Ⅲ组与Ⅰ、Ⅳ组相比差异无统计学意义（Pgt;0.05）。结论 5-HT神经细胞移植对慢性不可预见性抑郁症大鼠多器官损伤具有改善作用,其机制可能与移植细胞释放5-HT，调节了中枢至周围神经的胆碱能抗炎通路，并促进了周围器官的5-HT合成有关。 【关键词】 5-羟色氨； 细胞移植；抑郁症；氟西汀 The effect and possible mechanisms with transplantation of 5-HT(hydroxytryptamine)cell for the multiorgan lesions on the rats by chronic unpredictable depression 　　【Abstract】 Objective To explore the effect and mechanisms with transplantation of serotonergic neurons on rats by chronic unpredictable stress. Methods 40 male rats (purchased from the experimental animal center of Second Military Medical University) were randomly divided into four groups: control group (Ⅰ), chronic stress group (Ⅱ), chronic stress model transplanted stress cells (Ⅲ), and chronic stress model treated with fluoxetine (Ⅳ). The model induced with the chronic unpredictable stress, Ⅲ group were transplanted the serotonergic cells once into the subarachnoid space of the rats, Ⅳgroup were administered once a day for 3 weeks; All animals of each group measured behaviorally, observed anatomically and analyzed statistically using HE and immunohistochemical method. Results （1）compared with Ⅱ group, the behaviour of the rats in Ⅲ and Ⅳ groups improved obviously；（2）the gross anatomy shown：the lungs of the rats in Ⅱ group appeared focal congestion, 30％ suffered from the pulmonary abscess；the lesions of the lung in Ⅲ group were reduced，none of them was found the pulmonary abscess;（3）HE and immunohistochemical staining : the gastric gland cell presented atrophy and a numbers of t