AE-941抑制鼠视网膜新生血管实验探究.docVIP

AE-941抑制鼠视网膜新生血管实验探究 　【摘要】 目的 探讨AE-941(CARTCELL卡特消)对视网膜新生血管形成的抑制作用。方法 将鼠龄为7天的Wistar幼鼠置于75％浓度的氧环境中连续生活5天，建立高氧诱导的血管增生性视网膜病变幼鼠模型。随机分为正常对照、给氧对照组及治疗组（幼鼠玻璃体腔内注射AE-941 0.25 mg，0.5 μl，1 mg/ml，对侧眼注射相同体积的生理盐水作为对照）。采用ADP酶组织化学法行视网膜铺片，了解视网膜血管改变；用组织切片观察并计数突破视网膜内界膜的血管内皮细胞核数目，观察卡特消对视网膜新生血管形成的抑制情况。结果 给氧对照组（鼠龄17天）可见视网膜血管分布紊乱、密度增高，大量的视网膜新生血管，组织切片上可见较多突破视网膜内界膜的血管内皮细胞核。治疗组视网膜血管分布规则、密度减少，突破内界膜的内皮细胞细胞核数目明显减少，两组差异有显著统计学意义(Plt;0.001)。结论 AE-941可以抑制视网膜新生血管形成，有望成为防治血管增生性视网膜病变的一种有效的方法。 【关键词】 视网膜新生血管；卡特消 The inhibitory effects of AE-941 on retinal neovascularization ［Abstract］ Objective To explore the inhibitory effects of AE-941 on retinal neovascularization.Methods One-week-old Wistar mice were put into the environment with 75% oxygen for 5 days to establish models of vascular proliferation retinopathy.These mice were divided into normal control，high oxygen control and treatment groups(One eye of each mouse received an intravitreal injection of 0.25 mg，0.5 μl，1 mg/ml of AE-941，and the same volume of 0.9％ NaCl was injected into the other eye of the mouse both in these two groups as a control).The ADPase histochemical straining was used for retinal flatmount to observe changes of retinal vessels.The inhibitory effects of AE-941 on retinal neovascularization were evaluated by counting the endotheliocyte nuclei of new vessels extending from retina to vitreous in the tissue-slice.Results Disorganized distribution and high density of retinal vessels and retinal neovascularization of 17-day old mice in the high oxygen control;regular distributions and reduced density of retinal blood vessels in eyes in the treatment group were found in retinal flatmount.The number of the endotheliocyte nuclei of new vessels extending from retina to vitreous was less in the eyes in the treatment group than which in control group (P＜0.001).Conclusion Retinal neovascularization can be inhibited by intravit real injection of AE-941 which suggest that intravitreal injection of AE-941 may have potential theraputic benif