IL-6及人工关节假体周围骨溶解相关性.docVIP

IL-6及人工关节假体周围骨溶解相关性 　 作者：杨胜武，李悦，李晓阳，郑晓群，朱雄白，高建清 【摘要】 目的:探讨人工关节假体骨溶解模型动物外周血清和关节液中IL-6与骨溶解的相关性。 方法:健康成年新西兰兔40只，随机分为实验组三组及对照组，各10只。于动物右股骨髁间窝钻孔，股骨髓腔内置入1枚直径3 mm、长15 mm的不锈钢圆针。术后6周开始向三个实验组动物右侧膝关节腔内注入聚乙烯悬液0.5 mL；对照组注入等量生理盐水作为对照。每2周注射1次，共5次，分别于第16、18、20周处死三组实验动物，第20周处死对照组动物，分别取得标本液。ELISA法检测不同时间段外周血清和关节液中IL-6的含量。结果:在不同时间段，各组外周血清中IL-6含量的差异无显著性，各组关节液中IL-6含量的差异有显著性(P lt;0.05)，并随着实验时间的延长而增加。结论:在兔骨溶解动物模型中，动物外周血清IL-6水平在骨溶解过程中没有受到影响，而关节液中的IL-6水平随着骨溶解的进展而升高。 【关键词】 人工关节；聚乙烯微粒；骨溶解；白细胞介素-6；动物模型 Abstract: Objective: To assess the correlation between IL-6 and periprosthetic osteolysis in rabbit experimental study. Methods: Forty rabbits were randomly divided into four groups， 3 experimental groups and 1 control. For each rabbit， a steel nail with 3 mm in diameter and 15 mm in length was put in the medullary cavity of right femur from intercondylar fossa. Six weeks later， the 3 experimental groups were injected with 0.5 ml fluid containing with polyethylene particles in knee cavity， and the control group was injected with normal saline only， once per two weeks and 5 times totally. The 3 experimental groups were killed after 16，18，and 20 weeks，respectively，and the control group was killed after 20 weeks. Specimen fluids were obtained from peripheral circulation and joint cavity. The level of IL-6 in specimen fluids were measured by enzyme-linked immunoabsorbent assay. Results: For different duration， the level of IL-6 in peripheral blood showed no significant difference in every groups (P gt;0.05)， while the level of IL-6 in joint fluid was different significantly among groups (P lt;0.05)，and increased significantly with the time of implantation. Conclusion: In rabbit model of periprosthetic osteolysis， serum level of IL-6 is not affected in the process of ostoelysis， on the contrary， the level of IL-6 in joint fluid increases with progress of osteolysis. Key words: prosthesis；polyethylene particles；osteolysis；Interleukin-6；animal experiment 人工关节置换术已经是一种十分成熟的治疗手段，但假体的无