IL12+重组卡介苗对新生期小鼠脾脏T细胞功能亚群发育影响.docVIP

IL12+重组卡介苗对新生期小鼠脾脏T细胞功能亚群发育影响 　 作者：何云, 刘恩梅*, 杨锡强, 李欣, 刘玮 【摘要】 　　目的: 研究IL12+重组BCG新生期接种对小鼠脾脏T细胞功能亚群发育的影响。 方法: 新生清洁级BALB/c小鼠24只, 分对照组、 BCG组和IL12+重组BCG组。新生期接种, 4周后取脾细胞用流式细胞术检测CD3+CD8+IFNγ+、 CD3+CD8-IFNγ+、 CD3+CD8+IL４+、 CD3+CD8-IL４+、 CD4+Foxp3+ T细胞的比例, 其分别代表Tc1、 Th1、 Tc2、 Th2和调节性T细胞亚群。 结果: BCG组、 IL12+重组BCG组Th1、 Tc1细胞比例明显高于对照组（Plt;0.05或Plt;0.01）, BCG组与IL12+重组BCG组比较没有统计学意义; Tc2、 Th2和调节性T细胞比例各组间比较没有统计学意义; 二接种组Th1/Th2、 Tc1/Tc2和总IFNγ/IL4比值明显高于对照组（Plt;0.05或Plt;0.01）; CD4+Foxp3+ T细胞比例各组间比较没有统计学意义(Pgt;0.05)。结论: 新生期BCG和IL12+重组BCG接种组接种均能促进Th1、 Tc1细胞亚群的发育, 提高Th1/Th2、 Tc1/Tc2比值, 但不能影响CD4+FoxP3+Treg细胞的比例。 【关键词】 卡介苗 白细胞介素12 T细胞亚群 调节性T细胞 　　［Abstract］ AIM: To explore the effects of recombinant Balillus CalmetteGuerin secreting IL12 on the development of spleen T cell subsets of neonatal BALB/c mice. METHODS: Twentyfour neonatal BALB/c mices were divided 3 groups: Control, BCG , rBCG groups, which inoculated with BCG and rBCG intraperiotoneally within 2-3 days after birth. Four weeks later, spleen cells of mice were isolated and the percentage of CD3+CD8+IFNγ+/CD3+CD8IFNγ+/CD3+CD8+IL４+/ CD3+CD8-IL４+/ CD4+Foxp3+ T cells , which represent Tc1/Th1/Tc2/Th2/Treg cells respectively were detected by flow cytometry at single cells level, and Th1/Th2, Tc1/Tc2 ratio were calculated. RESULTS: The percentages of Th1, Tc1 cell of BCGvaccinated mice, rBCG vaccinated mice were significant higher than control mice (Plt;0.01), and there was no difference between the two vaccinated groups. The ratio of Th1/Th2, Tc1/Tc2 and total IFNγ/IL４ of two vaccinated groups were higher than control group. The percentages of Treg cells were no difference among the three groups (Pgt;0.05). CONCLUSION: Neonatal BCG and rBCGvaccination can significantly increase Tc1 and Th1 cells and Th1/Th2, Tc1/Tc2 ratio in spleen cells. Neonatal BCG and rBCG vaccination may have an same effects on development of T cell subsets in neonatal BALB/c mice. The percentage of CD4+Foxp3+ T cells among