Vitamin D3对裸鼠乳腺癌生长影响.docVIP

Vitamin D3对裸鼠乳腺癌生长影响 　 作者：周玮，王加林，赵亚平，王昱，张勇 【关键词】 ,胆骨化醇 Effect of vitamin D3 on growth of breast cancer in nude mouse xenograft models 　　【Abstract】 AIM: To study the effect of Vit D3 on breast cancer in nude mouse xenograft models. METHODS: Each Balb/c nude mouse was injected subcutaneously with human breast cancer cell line MCF7. Two thousand units of Vit D3 was injected in mice thigh muscle every other day and mice were fed with tamoxifen (5 mg/kg) every day. Forty experimental mice were randomly divided into 4 groups: Vit D3 group, the mixed group of Vit D3 and tamoxifen, tamoxifen group and control group. After 4 weeks, the tumor volume was measured and serum calcium and phosphorus levels were assayed. Tumor cells were analyzed with flow cytometery to detect apoptosis and cell cycle. RESULTS: Serum calcium levels in Vit D3 group were higher than those in control group（2.54±0.05 vs 2.32±0.09 mmol/L, Plt;0.05）, but phosphorus levels were lower（1.78±0.13 vs 2.34±0.06 mmol/L, Plt;0.05）. The apoptosis rate (%) of tumor cells in Vit D3 group was higher than that of control group （31.5±6.9 vs 7.8±2.5, Plt;0.05） and tumor cells were arrested in G0/G1 phase（50.0±3.2 vs 34.2±4.7, Plt;0.05）, especially in the mixed group treated with Vit D3 and tamoxifen. CONCLUSION: Vit D3 has some synergic effect with tamoxifen and can significantly induce apoptosis of breast cancer cells and arrest tumor cells in G0/G1 phase. 　　【Keywords】 cholecalciferol; breast neoplasms; apoptosis; cell cycle 　　【摘要】 目的： 探讨维生素D3（Vit D3）对乳腺癌裸鼠移植模型的治疗作用. 方法： 裸鼠皮下接种乳腺癌细胞MCF7，建立乳腺癌移植模型. Balb/c裸鼠共40只，随机等分为4组： 对照组、Vit D3 组、三苯氧胺(TAM)组、Vit D3 + TAM组. Vit D3 2000 u, im，隔日1次，三苯氧胺每日5 mg/kg, ig. 治疗4 wk后，检测肿块大小、血清钙、磷水平，并用流式细胞仪检测细胞凋亡和细胞周期. 结果： Vit D3 治疗组血清钙水平高于对照组（2.54±0.05 vs 2.32±0.09 mmol/L, P＜0.05），血磷水平低于对照组（1.78±0.13 vs 2.34±0.06 mmol/L, P＜0.05），Vit D3 使肿瘤细胞凋亡率(%)增加（31.5±6.9 vs 7.8±2.5, P＜0.05），并使细胞阻滞在G0/G1期（50.0±3.2 vs 34.2±4.7, P＜0.05），与TAM有协同作用. 结论： Vit D3 体内可明显诱导乳腺癌细胞凋亡并使细胞阻滞在G0/G1期，增强TAM抗肿瘤作用. 　　【关键词】 胆骨